2 The membrane from Ganoderma residue(SACCHACHITIN)effects on the growth of keratinocytes and matrix metalloproteinases (MMPs)SACCHACHITIN membrane, prepared from the waste residue of the fruiting body of Ganoderma taugae, was used in our previous study for the enhancement of skin wound healing in animal models. In the present study, the membrane was estimated for its effects on the growth of keratinocytes and the activity of matix metalloproteinases (MMPs) as well as healing of the skin wounds on human. Fresh human foreskin was employed as the source of the keratinocyte culture and a modified keratinocyte-SFM medium supplemented with 0.2ng/ml recombinant epidermal growth factor and 30mg/ml bovine pituitary extract was used to enhance a successful growth of keratinocytes under an atmosphere of 5% CO2, at 37℃. The results indicated that 0.01% of SACCHACHITIN enhanced the proliferation of keratinocytes in the culture on the 4th and 5th day, and the cells showed neither morphological alteration nor disorder in proliferation. These evidences elucidated that SACCHACHITIN was not cytotoxic and safe toward the growth of keratinocytes. Thus, SACCHACHITIN could play a positive role in the proliferation and differentiation of keratinocytes around the wounds and the accelerated wound healing of epidermal tissue. In addition, the microscopic observation during the growth of keratinocytes showed that normal proliferation and differentiation took place along the margin of SACCHACHITIN membrane. These could indicate that SACCHACHITIN was cytocompatible for keratinocytes. Electrophoresis analysis and inhibition tests for binding effect of SACCHACHITIN on MMPs showed that SACCHACHITIN reduced the MMPs in extracellular matrix degradation around the wounds, facilitate establishment of extracellular matrix around the wounds, and these resulted in rapid wound healing. SACCHACHITIN was used as a skin dressing for the patients who suffered unhealing skin for at least over 7 months. The preliminary clinical observation showed the wound turns better and starts to heal. The analysis of MMPs on the tissue by ELISA of wound indicated a significant decrease of MMP level.