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Bioprocess Engineering
養菌&bioreactor Bioprocess Engineering 基因重組﹝PCR, host selection﹞ 或微生物篩選﹝plasmid selection﹞ Lab. 生產 工廠生產 前處理﹝殺菌等﹞ 生化反應器 破碎細胞 分離及純化 Recombinant protein metabolites 細胞固定化﹝酵素固定化﹞
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﹝一﹞前處理 殺菌: 高溫高壓﹝125℃ ~140℃ ﹞ heat sterilizer, auto clave
化學藥品 酒精,detergent﹝SDS…等﹞ 過濾
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colony
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Isolation of single colony
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Antibiotics resistance
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rich media defined media Determining cell mass dry weight optical density (O.D.) spectrophotometer O.D. 550 O.D. 600
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﹝二﹞生化反應器 Fermentor 發酵槽﹝batch﹞ chemostat
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Chemostat: cellular growth is usually limited by one essential nutrient, and other nutrients are in excess (P183,184)
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Turbidostat: The cell concentration in the culture vessel is maintained constant by monitoring the optical density of the culture and controlling the feed flow pate. (P184)
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The ideal chemostat:
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3.Operability and reliability 4.Market economics
使用chemostat為bioreactor,其產量將遠大於batch。可是為什麼工業界使用batch的比率遠高於chemostat? 1.Secondary products 2.Genetic instability 3.Operability and reliability 4.Market economics
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