生物結構期末報告 學生:葉雅如 M93360018 老師: 鄒文雄 教授.

Presentation on theme: "生物結構期末報告 學生:葉雅如 M93360018 老師: 鄒文雄 教授."— Presentation transcript:

1 生物結構期末報告 學生:葉雅如 M 老師: 鄒文雄 教授

2 Aim 分析其他蛋白質與p25/26是否有保留性序列存在,是否有演化上的關係存在

3 Introduction CFTR , A chloride channel
The cystic fibrosis transmembrane conductance regulator (CFTR) is a 1480 amino acid membrane bound glycoprotein ABC-ATPase proteins. two, six span membrane bound regions(TMS1,TMS2), ATP binding domain. The R-domain is a unique feature of CFTR within the ABC superfamily. The model shows the two transmembrane-spanning domains (TMS1 and TMS2), the two nucleotide binding domains (NBF1 and NBF2), and the regulatory domain (R). The location of NBF1 peptides P25 and P26 used in this study is also indicated. Biochemistry, 38 (23), , /bi S (99)

4 P26/P25 1CKY Compound Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator (Cftr); Chain: A; Fragment: P26, F508 Mutation Region; Synonym: P26 In H2O; Engineered: Yes 1CKZ Compound Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator (Cftr); Chain: A; Fragment: P25, F508 Mutation Region; Synonym: P25 In H2O; Engineered: Yes

5 NBD1 1XMJ Compound: Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator; Chain: A; Fragment: Nucleotide Binding Domain One; Synonym: Cftr; Camp- Dependent Chloride Channel; Ec: ; Engineered: Yes; Mutation: Yes

6 利用NBD1作protein and protein BLAST

7

8 任選出四條不同E-value序列 >gi| |ref|NP_ | hypothetical protein AGR_C_4902 [Agrobacterium tumefaciens str. C58] >gi| |pdb|1JSQ|A Chain A, Structure Of Msba From Escherichia Coli: A Homolog Of The Multidrug Resistance Atp Binding Cas >gi| |ref|NP_ | hypothetical protein AGR_L_1532 [Agrobacterium tumefaciens str. C58] >gi| |ref|NP_ | putative ABC transporter protein, fused ATP-binding and membrane-spanning components, secretes ExpE1 [Sinorhizobium meliloti 1021]

9 Scores Table (7個序列)

10 Alignment

11 討論 由於P25/26序列太短無法直接作protein-protein BLAST
使用NBD1(P25)作搜尋不易找出於與P25/26相對位置相同的protein 故無法做進一步探討 但與NBD1相似性高的序列,多屬於各種ATPase-family的膜蛋白,似乎也可推知膜蛋白的具有Domain彼此具有相當的同源性

12 THE END