Bioprocess Engineering

Presentation on theme: "Bioprocess Engineering"— Presentation transcript:

1 Bioprocess Engineering
養菌&bioreactor Bioprocess Engineering 基因重組﹝PCR, host selection﹞ 或微生物篩選﹝plasmid selection﹞ Lab. 生產 工廠生產 前處理﹝殺菌等﹞ 生化反應器 破碎細胞 分離及純化 Recombinant protein metabolites 細胞固定化﹝酵素固定化﹞

2 ﹝一﹞前處理 殺菌： 高溫高壓﹝125℃ ~140℃ ﹞ heat sterilizer, auto clave
化學藥品 酒精，detergent﹝SDS…等﹞ 過濾

3 colony

4 Isolation of single colony

5 Antibiotics resistance

6 rich media defined media Determining cell mass dry weight optical density (O.D.) spectrophotometer O.D. 550 O.D. 600

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10 ﹝二﹞生化反應器 Fermentor 發酵槽﹝batch﹞ chemostat

11 Chemostat: cellular growth is usually limited by one essential nutrient, and other nutrients are in excess (P183,184)

12 Turbidostat: The cell concentration in the culture vessel is maintained constant by monitoring the optical density of the culture and controlling the feed flow pate. (P184)

13 The ideal chemostat:

14 3.Operability and reliability 4.Market economics
使用chemostat為bioreactor，其產量將遠大於batch。可是為什麼工業界使用batch的比率遠高於chemostat？ 1.Secondary products 2.Genetic instability 3.Operability and reliability 4.Market economics