川北医学院 / North Sichuan Medical College 光学体视框演示: 长形精子细胞核计数 Demonstration of the optical disector: counting of the elongated spermatid nuclei 成年大鼠睾丸细胞核辨认 Identification of nuclei in the adult rat testis 杨正伟 / Zheng-Wei Yang 川北医学院 / North Sichuan Medical College 2013年4月 / April 2013

图注：成年大鼠睾丸切片（25 m厚，甲基丙烯酸树脂包埋）内 连续光学切片 a-u（总高 10 m），4 个禁线框（每个 ~200 m2）。 Figures legend: Serial optical sections a-u (total height 10 m) within an adult rat testicular section (25 m in thickness, methacrylate-embedded), 4 forbidden-line frames (each ~200 m2)

根据光学体视框*，从平面（光学切片）a至u计数新出现的，只计数长形精子细胞核，有下列 3 个细胞核应被计数：/ According to the optical disector*, counting the newly appearing from plane (optical section) a to u, and only counting elongated spermatid nuclei, the following 3 nuclei should be counted: *杨正伟. 生物组织形态定量研究基本工具: 实用体视学方法. 北京: 科学出版社, 2012. 94-99, 193-200 / Yang ZW. Practical Stereological Methods: Essential Tools for Morphometric Studies of Biological Tissues. Beijing: Science Press; 2012. pp 94-99, 193-200 [Book in Chinese]

平面e上箭头所指细胞核。该核的轮廓（断面）从平面c开始出现（聚焦），其左端（）刚好与禁线框的计数线相交。 The nucleus pointed by arrow  on plane e. The profiles (sections) of the nucleus start to appear (be in focus) on plane c, and its left end () just intersects the counting line of the forbidden-line frame.

平面o上箭头所指细胞核。该核的轮廓从平面m开始出现。/ The nucleus pointed by arrow  on plane o 平面o上箭头所指细胞核。该核的轮廓从平面m开始出现。/ The nucleus pointed by arrow  on plane o. The profiles of the nucleus start to appear (be in focus) on plane m. 平面u上箭头所指细胞核。该核的轮廓从平面t开始出现。/ The nucleus pointed by arrow  on plane u. The profiles of the nucleus start to appear (be in focus) on plane t.

切片上所见细胞核种类 / Types of nuclei seen on sections： LC：Leydig细胞 / Leydig cell EC：内皮细胞 / endothelial cell MC：肌样细胞 / myoid cell SC：Sertoli细胞 / Sertoli cell A：A型（或非B型）精原细胞 / type A (or non-type B) spermatogonia B：B型精原细胞 / type B spermatogonia PS：粗线期初级精母细胞 / pachytene primary spermatocytes RS：球形精子细胞 / round spermatid ES：长形精子细胞 / elongated spermatid

Slow animated cartoon demonstration (play this slide) 慢速动画演示（放映该页幻灯） Slow animated cartoon demonstration (play this slide)

Fast animated cartoon demonstration (play this slide) 快速动画演示（放映该页幻灯） Fast animated cartoon demonstration (play this slide)