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Side Chain Assignment (H)C(CO)NH-TOCSY: Side Chain C chemical shifts
生物大分子波谱学原理 吴季辉 Side Chain Assignment (H)C(CO)NH-TOCSY: Side Chain C chemical shifts H(CC)(CO)NH-TOCSY: Side chain H chemical shifts HCCH-COSY: adjacent C-C correlation HCCH-TOCSY: long range C-C correlation within one residue
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生物大分子波谱学原理 吴季辉 CCONH-TOCSY
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生物大分子波谱学原理 吴季辉 HCONH-TOCSY
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生物大分子波谱学原理 吴季辉 13C－13C相关谱 碳－碳相关谱 HCCH-COSY（1H-13C-13C-1H correlation spectroscopy）及HCCH-TOCSY（1H-13C-13C-1H total correlation spectroscopy）用于证认13C标记蛋白质的脂肪链1H及13C，信号传递是从1H开始，经过单键J偶合到13C，再通过类似同核COSY及TOCSY的机制传递到其他13C，最后通过单键J偶合回传到1H。所以这类实验可以看作1H同核COSY及TOCSY在13C上的推广。除了可以获得13C的信息，也可得到1H的信息，对于大蛋白质而言，效果比1H同核COSY及TOCSY更好，因为在1H同核COSY及TOCSY中起作用的是比较小的1H同核J偶合（一般小于10Hz），而这里虽有三步传递，却均是比较大的J偶合，其中JHC约140Hz，JCC约35Hz。因此这类实验对于大蛋白质的侧链1H，13C的证认非常重要。
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生物大分子波谱学原理 吴季辉 13C－13C相关谱
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉
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C－13C相关谱 生物大分子波谱学原理 吴季辉
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生物大分子波谱学原理 吴季辉 C－13C相关谱
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生物大分子波谱学原理 吴季辉 C－13C相关谱
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生物大分子波谱学原理 吴季辉 C－13C相关谱
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C－13C相关谱 生物大分子波谱学原理 吴季辉
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Conventional implementation: 1H magnetization is transferred in a refocused INEPT to 13C in-phase magnetization. After C,C-TOCSY mixing, the back-transfer is achieved in an analogous manner. The incrementation of delays required for shared-time evolution in t1 is also indicated. Note that the C' 180 pulse during the t1 evolution period is not absolutely required, because 2JH,C' couplings are small.
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Doubly sensitivity-enhanced 3D H(C)CH-TOCSY experiment with hetero-nuclear gradient echo for solvent suppression. The heteronuclear in-phase transfer is implemented with a yxz-ICOS-CT sequence. Four different combination of gradients and and of phase settings and (corresponding to echo/antiecho pathways for both indirect dimensions) have to be recorded and stored in different memory locations. Modifications required for a semi-constant-time evolution during t1 are indicated. The operator F is the sum of I operators denoting the proton spins
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