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中研院生物醫學研究所 譚婉玉老師實驗室 生科05 劉泰安 U901671
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Splicing Exon 1 Exon 2 Intron Pre-mRNA mRNA
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5` 3`
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SF1 U2AF U2AF: U2-associated factor
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暑假的工作 確認U2AF35, U2AF65和WW domain的 相對位置
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Translate U2AF35 and U2AF65 U2AF35 or U2AF65 DNA template
NTP, amino acid (minus Met) Rabbit Reticulocyte Lysate RNA polymerase Other buffer [35S]Met 30℃, 90mins
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Binding GST protein Protein for translation GST-CA150WW2 protein
GST-FBP11WW protein 30℃, 30mins
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Pulled down Solution which made before beats 4℃, 2hours
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cassette 2× screen film -70℃, 8hours Gel on paper
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U2AF65 U2AF35 SF1 U35 10% input U65 10% input GST GST- CA GST- FB U2AF65 U2AF35?
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U2AF35 U2AF35 U2AF65 SF1 U2AF65 U35 10% input U65 10% input GST- FB GST- CA GST GST GST- CA GST- FB U2AF35 ? U2AF65
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A model fits my result
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謝謝大家收聽 下星期同一時間請準時收看 本節目將邀請到 楊永壽及陳碩璜同學
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