第一部分：以矽膠管柱搭配含水移動相之高壓液相層析法分析化妝品與藥品中陽離子殺菌劑成分。第二部分：以HPLC方法分離並鑑別黏膜用化妝品（口紅）中之非法煤焦色素。 第一部分：以矽膠管柱搭配含水移動相之高壓液相層析法分析化妝品與藥品中陽離子殺 菌劑成分 本研究提供一簡單、快速、可靠且與傳統使用逆相分析不同之高壓液相層析.

Presentation on theme: "第一部分：以矽膠管柱搭配含水移動相之高壓液相層析法分析化妝品與藥品中陽離子殺菌劑成分。第二部分：以HPLC方法分離並鑑別黏膜用化妝品（口紅）中之非法煤焦色素。 第一部分：以矽膠管柱搭配含水移動相之高壓液相層析法分析化妝品與藥品中陽離子殺 菌劑成分 本研究提供一簡單、快速、可靠且與傳統使用逆相分析不同之高壓液相層析."— Presentation transcript:

1 第一部分：以矽膠管柱搭配含水移動相之高壓液相層析法分析化妝品與藥品中陽離子殺菌劑成分。第二部分：以HPLC方法分離並鑑別黏膜用化妝品（口紅）中之非法煤焦色素。
第一部分：以矽膠管柱搭配含水移動相之高壓液相層析法分析化妝品與藥品中陽離子殺 菌劑成分 本研究提供一簡單、快速、可靠且與傳統使用逆相分析不同之高壓液相層析 法，它可同時鑑別與定量化妝品中二種陽離子殺菌劑及藥品中四種陽離子殺菌劑。 本 分析法係採用矽膠層析管柱搭配含水移動相，偵測器為具紫外光262nm波段。以六種陽離 子殺菌劑不同濃度之溶液製作檢量線皆呈線性關係(r>0.999)。應用此分析法，不易受化 妝品或藥品賦型劑及其他成分之影響，其波峰尖銳、具對稱性，且操作簡單；適合四級胺 類藥品與化妝品之鑑別與定量。最低檢測濃度chlorhexidine gluconate為0.2 (g/mL，其 餘5種約為2.5 (g/mL。benzalkonium chloride、chlorhexidine gluconate添加於化妝品 中其回收率皆大於95%，而domiphen bromide，benzethonium chloride，cetylpyridiniu m chloride與dequalinium chloride其回收率第二部分：以HPLC方法分離並鑑別於黏膜用 化妝品(口紅)中之非法煤焦色素摘要 非法添加於黏膜化妝品規定外煤焦色素係具有強 毒性或致癌性，依據我國國家標準(CNS)，化妝品中有機性著色劑之檢驗方法係採用濾紙 層析法與薄層層析法。本法採用HPLC方法用以分離並鑑別黏膜化妝品中摻加之非法煤焦色 素，以提高檢驗效率及精確度。 高效液相層析分析條件：層析管為Cosmosil 5C18-AR -Ⅱ。移動相：(a)水溶性煤焦色素為0.07M醋酸銨(包含0.01M 溴化四丁基銨：乙：甲醇 =55：35：10，可分離16種色素。(b)脂溶性煤焦色素為甲醇：水=95：5，可分離6種色素 。偵測器為具有可見光波長之光電二極體矩陣檢測器。最小偵測濃度為0.05(g/mL，22種 非法之煤焦色素均可得到良好之分離效果。

2 Ⅰ: Simultaneous Assay of Cationic Disinfectant in Cosmetics and Pharmaceutical Products Using HPLC Equipped with a Silica Gel Column and an Aqueous Mobile Phase. Ⅱ: HPLC Separation and Identificatio Ⅰ. Simultaneous Assay of Cationic Disinfectant in Cosmetics and Pharmaceutica l Products Using HPLC Equipped with a Silica Gel Column and an Aqueous Mobile PhaseThis report reveals that a simple, rapid and reliable high performance li quid chromatographic (HPLC) method has been developed. This method provides si multaneously to identify and quantify two cationic disinfectants: benzalkonium chloride and chlorhexidine gluconate in cosmetics; as well as four other cati onic disinfectants: domiphen bromide, benzethonium chloride, cetylpyridinium c hloride and dequalinium chloride in pharmaceuticals. The HPLC analytical metho d involves the use of a silica gel column and an aqueous mobile phase. The dis infectants are separated by isocratic chromatography on a m-porasil silica gel column (3.9 * 300 nm) using an UV detector at 262 nm. Six standard solutions of the cationic disinfectants with different concentrations were found to have excellent linear relationships (r > 0.999). The results obtained are not inf luenced by the vehicle incorporated in cosmetics or pharmaceuticals by using this analytical meⅡ. HPLC Separation and Identification of Illegal Coal Tar D yes in Mucous Cosmetics Illegal coal tar dyes blended in mucous cosmetics cou ld induce toxic and carcinogenic threats. In accordance with Chinese National Standard, the methods of hygienic test adopted for analyzing organic coloring materials in cosmetics are paper chromatography (PC) and thin layer chromatogr aphy(TLC). This study was using HPLC method to promote the analysis efficienc y in separating and identifying the illegal coal tar dyes in mucous cosmetics. The HPLC column was Cosmosil 5C18-AR-Ⅱ,mobile phase comparitions were as fo llows:(a) for hydrophilic coal tar dyes :0.07M ammonium aceate(including 0.01M tetrabutylammonium bromide ) : acetonitrile : methanol=55:35:10. This method is capable of separating 16 coal tar dyes. (b) for hydrophobic coal tar dyes, methanol:water=95:5, a total of 6 coal tar dyes could be separated. Visible wa velength photodiode array detector was used for detection. The minimum detecta ble concentration was 0.05μg/mL. The results clearly shows that a total of 2 2 illegal coal tar dyes can be separated and identified successfully as report ed above.