Journal of Proteome Research (PMID: 26709725; IF= 4.173) Proteome differences between hepatitis B virus genotype B and genotype C induced hepatocellular carcinoma revealed by iTRAQ based quantitative proteomics Journal of Proteome Research (PMID: 26709725; IF= 4.173) 报告人：董浩 IMI CONFIDENTIAL

Background 分类：（基于LC-MS） 定性 定量 标记：SILAC、iTRAQ、 ICAT 非标记 bla 蛋白质组学（proteomics）是从整体角度分析细胞内蛋白质组成、表达变化、修饰状态，了解蛋白质之间的相互作用，从蛋白水平揭示蛋白质功能与细胞生命活动规律。 分类：（基于LC-MS） 定性 定量 标记：SILAC、iTRAQ、 ICAT 非标记 其研究内容主要包括：鉴定特定细胞、组织或器官的蛋白质种类（蛋白质组全谱鉴定）、特定条件下蛋白质的表达量变化研究（定量蛋白质组学）、明确蛋白质在生命活动中执行的功能（功能蛋白质组学）、揭示蛋白质之间的复杂相互作用机制（相互作用蛋白质组学）、描绘蛋白质的精确二维、三维以致四维结构（结构蛋白质组学）、以及蛋白质翻译后修饰研究（修饰蛋白质组学）。 IMI CONFIDENTIAL bla

Background 定量蛋白质组学 标记（label）定量：用不同的化学试剂或同位素作为标记物，对不同样品进行区别标记，然后混合、经过LC-MS/MS分析。 方法：SILAC：标记效率高，但只能针对细胞。 ICAT：兼容性广，但只能对含有半胱氨酸的肽段标记，且只能对两个样品标记。 iTRAQ：标记效率高、分离能力强、分析范围广、自动化程度高、定量结果准确。 IMI CONFIDENTIAL

Background 蛋白质组学结果分析 差异表达蛋白的种类与定量 生物信息学分析 GO分析：生物学过程、细胞组分、分子功能 KEGG分析：信号通路相关 聚类分析：展示作用 PPI分析：蛋白互作 IMI CONFIDENTIAL

Background 乙型肝炎病毒(HBV)是肝细胞癌的主要病因，HBV基因型B和C基因型是最普遍的。不同的病毒基因型对肝细胞癌的临床结果具有不同影响。然而,潜在的分子机制差异并不清楚。 目的：通过蛋白质组学方法分析 B、C基因型HBV引发的肝癌中蛋白质组差异，研究二者对肝癌发生的不同影响。 IMI CONFIDENTIAL

Materials and Methods 分组：B型癌旁组织、 B型癌组织 C型癌旁组织、 C型癌组织 Website Database: GO分析：Avadis、BiNGO、DAVID软件 通路分析：IPA、MetaCore软件 Methods: LC-MS/MS Analysis（iTRAQ） Western Blotting Quantitative Real-time PCR IMI CONFIDENTIAL

Results Clinical Characteristics of the Study Population IMI CONFIDENTIAL

Results 2. Quantitative Proteomics of the HBV Genotype-B- and Genotype-C-Induced HCC IMI CONFIDENTIAL

Results 2. Quantitative Proteomics of the HBV Genotype-B- and Genotype-C-Induced HCC IMI CONFIDENTIAL

Results 3. Identification of the Differentially Expressed Proteins between HBV Genotype-B- and Genotype-C-Induced HCC IMI CONFIDENTIAL

Results 3. Identification of the Differentially Expressed Proteins between HBV Genotype-B- and Genotype-C-Induced HCC IMI CONFIDENTIAL

Results 3. Identification of the Differentially Expressed Proteins between HBV Genotype-B- and Genotype-C-Induced HCC IMI CONFIDENTIAL

Results 3. Identification of the Differentially Expressed Proteins between HBV Genotype-B- and Genotype-C-Induced HCC IMI CONFIDENTIAL

Results 3. Identification of the Differentially Expressed Proteins between HBV Genotype-B- and Genotype-C-Induced HCC These results also proved that there might be different molecular backgrounds and molecular machanisms between genotype-B-induced HCC and genotype-C-induced HCC. IMI CONFIDENTIAL

Results 4. IPA Network Analysis of the Differentially Expressed Proteins surrounding noncancerous tissue: inflammation survival proliferation of tumor cells IMI CONFIDENTIAL

Results 4. IPA Network Analysis of the Differentially Expressed Proteins cancerous tissues: lipid metabolism molecular transport Therefore, different HBV genotype-induced HCC should have its own specific molecular characteristics and molecular mechanisms IMI CONFIDENTIAL

Results 5. Verification of the Differential Expression of ARFIP2 and ANXA1 ARFIP2, regulate NF-κB signaling, only downregulated in surrounding noncancerous, ANXA1, anti-inflammatory, only upregulated in cancerous tissues Therefore, they might be potential biomarkers to distinguish the HBV genotype-B- and genotype-C-induced HCC IMI CONFIDENTIAL

Results 5. Verification of the Differential Expression of ARFIP2 and ANXA1 IMI CONFIDENTIAL

Results 5. Verification of the Differential Expression of ARFIP2 and ANXA1 IMI CONFIDENTIAL

bla 临床样本 Overall, we have applied the iTRAQ-based quantitative proteomics approach to compare the protein expression profile alternations of HBV genotype-B- and genotype-C-induced HCC, and identified potential biomarkers and possible therapeutic targets for the HBV genotypes B- and C-induced HCC. LC-MS/MS（iTRAQ） GO-annotation pathway analysis 在2个小时时影响大。 验证 IMI CONFIDENTIAL bla

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