Long- and short-day control of flowering time in Arabidopsis

Presentation on theme: "Long- and short-day control of flowering time in Arabidopsis"— Presentation transcript:

1 Long- and short-day control of flowering time in Arabidopsis
植物生理學實驗 12 長、短日照對阿拉伯芥開花時間影響 Long- and short-day control of flowering time in Arabidopsis 授課教授：傅士峯老師 助教：呂學宇、張凱雅 值日組：G10 104/12/16

2 實驗目的 觀察阿拉伯芥在不同光照時間之下，開花時植株葉片有何不同

3 實驗原理

4 Effects of environmental cues and hormones on flowering time control
TEMP VRN LIGHT HORM REP Annu. Rev. Genet. 2003

5 Effects of environmental cues on flowering time control
Flowering time control by different photoperiods Garner and Allard, 1920 Long-Day Short-Day Adapted from : Adapted from Alex Webb lab, Department of Plant Sciences, University of Cambridge

6 Effects of environmental cues on flowering time control
Flowering time control by temperature fluctuation 不經一番寒澈骨 焉得梅花撲鼻香 Photo: Fu SF Adapted from Alex Webb lab, Department of Plant Sciences, University of Cambridge

7 Effects of low temperature on flowering in Brassica plants Vernalization
-V V +V V Cabbage Arabidopsis Brassicaceae +V V The Plant Cell 2004

8 Effects of multiple environmental cues on flowering
Photoperiod and vernalization Arabidopsis Wild-type Early-flowering strain Wild-type Winter-annual strain Plant, Cell and Environment 2000

9 Leaf-produced floral signals-Flowering Locus T (FT)
FT protein but not FT mRNA is phloem mobile 2009 Current Opinion in Plant Biology

10 Flowering locus C (FLC) is a negative regulator of flowering
time control and mediated by vernalization

11 _ + Flowering time control in Arabidopsis (Col)
FLC-negative regulator ; FT-positive regulator Control _ + 35S::AtFLC Data: SF Fu

12 Arabidopsis development—Adult and juvenile

13 Leaf trichomes—Adult and juvenile
Juvenile Juvenile Adult Adult Adult Adult

14 Leaf numbers at bolting-Example

15 Leaf numbers at bolting-Example

16 實驗步驟 將葉片依序由下而上剪下，並照順序排列。 奇數組：short day 偶數組：long day 1 2 3 4 5 6 7 8 13
9 10 11 12

17 實驗結果 結果一： 計算負責植株的葉片數量。 與合作組交換數據後，將數據繪製成長條圖。 Short day Long
No. of leaves to bolting 1 2 3 4 5 6 7 8 13 9 10 11 12

18 實驗結果 結果二： 觀察葉片葉面與葉背絨毛分佈，並繪製成圖。 Adult Juvenile
註：除了自己組別負責的植株外，合作組的植株也要觀察記錄 Adult Juvenile

19 問題與討論 葉片的成熟與葉片的數量是否與光照時間有相關？

20 報告繳交 報告格式： 1.實驗目的 2.實驗原理 3.實驗結果 4.問題與討論 5.補充資料 6.心得
繳交期限：下周三(12/23)課程結束前