Bioprocess Engineering 養菌&bioreactor Bioprocess Engineering 基因重組﹝PCR, host selection﹞ 或微生物篩選﹝plasmid selection﹞ Lab. 生產 工廠生產 前處理﹝殺菌等﹞ 生化反應器 破碎細胞 分離及純化 Recombinant protein metabolites 細胞固定化﹝酵素固定化﹞
﹝一﹞前處理 殺菌: 高溫高壓﹝125℃ ~140℃ ﹞ heat sterilizer, auto clave 化學藥品 酒精,detergent﹝SDS…等﹞ 過濾
colony
Isolation of single colony
Antibiotics resistance
rich media defined media Determining cell mass dry weight optical density (O.D.) spectrophotometer O.D. 550 O.D. 600
﹝二﹞生化反應器 Fermentor 發酵槽﹝batch﹞ chemostat
Chemostat: cellular growth is usually limited by one essential nutrient, and other nutrients are in excess (P183,184)
Turbidostat: The cell concentration in the culture vessel is maintained constant by monitoring the optical density of the culture and controlling the feed flow pate. (P184)
The ideal chemostat:
3.Operability and reliability 4.Market economics 使用chemostat為bioreactor,其產量將遠大於batch。可是為什麼工業界使用batch的比率遠高於chemostat? 1.Secondary products 2.Genetic instability 3.Operability and reliability 4.Market economics