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Traditional Techniques
Immunizing a rabbit and bleeding Precipitation reaction Double diffusion Immunoelectrophorsis 說明文字
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紐西蘭白兔之免疫及採血 抗原免疫 耳朵採血 說明文字 莊榮輝 (1985) 博士論文. p.78 收集抗血清 蛋黃 IgY
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小白鼠免疫及腹水誘生流程 X mL (X = 1-10) 加佐劑製成乳劑 全採血 Ascites Fluids 腹水 2 4 6 8 10
Antigen (50 mg/mouse) Emulsified in 0.5 mL Freund's Complete Adjuvant 加佐劑製成乳劑 BALB/c 2 4 6 8 10 12 14 wk At least three booster shots, same dose in 0.5 mL Freund's Incomplete Adjuvant Booster shots might be reduced if TiterMax is use as adjuvant 全採血 Trial Bleeding Titer Determination Total Bleeding, < 1 mL Pristane (0.5 mL) 說明文字 NS-1 Cell (10 6 cells) Ascites Fluids X mL (X = 1-10) 腹水
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傳統的抗原及抗體沉澱反應 Immunoprecipitation
當量點 抗原過多 抗體過多 說明文字 Ballanti (1985) Immunology III. p.169
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雙向免疫擴散法 Double Diffusion
Roitt et al (2001) Immunology. P.134 Ballanti (1985) Immunology III. p.169 洋 菜 膠 片 說明文字
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+ - 免疫電泳膠片及電泳槽 Immunoelectrophoresis c c 染色脫色 說明文字
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