Long- and short-day control of flowering time in Arabidopsis 植物生理學實驗 12 長、短日照對阿拉伯芥開花時間影響 Long- and short-day control of flowering time in Arabidopsis 授課教授：傅士峯老師 助教：呂學宇、張凱雅 值日組：G10 104/12/16
實驗目的 觀察阿拉伯芥在不同光照時間之下，開花時植株葉片有何不同
實驗原理
Effects of environmental cues and hormones on flowering time control TEMP VRN LIGHT HORM REP Annu. Rev. Genet. 2003
Effects of environmental cues on flowering time control Flowering time control by different photoperiods Garner and Allard, 1920 Long-Day Short-Day Adapted from : http://tianwei.pixnet.net/blog/post/10853484 Adapted from Alex Webb lab, Department of Plant Sciences, University of Cambridge
Effects of environmental cues on flowering time control Flowering time control by temperature fluctuation 不經一番寒澈骨 焉得梅花撲鼻香 Photo: Fu SF Adapted from Alex Webb lab, Department of Plant Sciences, University of Cambridge
Effects of low temperature on flowering in Brassica plants Vernalization -V +V +V -V Cabbage Arabidopsis Brassicaceae +V -V The Plant Cell 2004
Effects of multiple environmental cues on flowering Photoperiod and vernalization Arabidopsis Wild-type Early-flowering strain Wild-type Winter-annual strain Plant, Cell and Environment 2000
Leaf-produced floral signals-Flowering Locus T (FT) FT protein but not FT mRNA is phloem mobile 2009 Current Opinion in Plant Biology
Flowering locus C (FLC) is a negative regulator of flowering time control and mediated by vernalization
_ + Flowering time control in Arabidopsis (Col) FLC-negative regulator ; FT-positive regulator Control _ + 35S::AtFLC Data: SF Fu
Arabidopsis development—Adult and juvenile
Leaf trichomes—Adult and juvenile Juvenile Juvenile Adult Adult Adult Adult
Leaf numbers at bolting-Example
Leaf numbers at bolting-Example
實驗步驟 將葉片依序由下而上剪下，並照順序排列。 奇數組：short day 偶數組：long day 1 2 3 4 5 6 7 8 13 9 10 11 12
實驗結果 結果一： 計算負責植株的葉片數量。 與合作組交換數據後，將數據繪製成長條圖。 Short day Long No. of leaves to bolting 1 2 3 4 5 6 7 8 13 9 10 11 12
實驗結果 結果二： 觀察葉片葉面與葉背絨毛分佈，並繪製成圖。 Adult Juvenile 註：除了自己組別負責的植株外，合作組的植株也要觀察記錄 Adult Juvenile
問題與討論 葉片的成熟與葉片的數量是否與光照時間有相關？
報告繳交 報告格式： 1.實驗目的 2.實驗原理 3.實驗結果 4.問題與討論 5.補充資料 6.心得 繳交期限：下周三(12/23)課程結束前