性别决定与表观遗传 陈德桂 中科院上海生科院生化与细胞所 cdchen@sibs.ac.cn 021-54921148
性别决定 Sex is determined by sex chromosome sex chromosome
SRY: Sex determination Region on the Y chromosome
Sufficiency: transgenic
表观遗传与个体发育 什么是表观遗传 与个体发育的关系 我是怎么进入表观遗传领域的 做了什么
遗传(genetics) and 表观遗传(epigenetics)
Epigenetic phenomena Polycomb and Trithorax Drosophila study Sex comb Not determined by DNA sequence
B + SAM-CH3 Histones + Enzyme
个体发育与表观遗传
Imprinted genes
Imprinting: allelic specific silencing
The Problem: Dosage difference X/Y contain different number of genes
When is X-chromosome inactivated?
Epigenetic regulation of X inactivation
Heterochromatin formation
Epigenetics in cancer
Epigenetics with iPS
表观遗传
遗传信息:可遗传的DNA系列 表观遗传信息:可遗传的非DNA系列信息 DNA修饰(甲基化) 非编码RNA 组蛋白修饰(甲基化等) 染色体重塑
DNA不变 如何形成各种细胞, 且每种细胞保持其遗传性?
Nature. 2009 Mar 18
Histone marked enhancers are cell type specific
Expression of cell-type specific genes associates with specific enhancers
Life cycle of C. elegans
Progressive reduction of brood size in spr-5 ko
Percentage of sterile animals
H3K4me2 retention in mutant animals
Spr-5 regulated oogenesis and spermatogenesis
Outcross abolished epigenetic memory
我怎么进入表观遗传学领域的?