中研院生物醫學研究所 譚婉玉老師實驗室 生科05 劉泰安 U901671
Splicing Exon 1 Exon 2 Intron Pre-mRNA mRNA
5` 3`
SF1 U2AF U2AF: U2-associated factor
暑假的工作 確認U2AF35, U2AF65和WW domain的 相對位置
Translate U2AF35 and U2AF65 U2AF35 or U2AF65 DNA template NTP, amino acid (minus Met) Rabbit Reticulocyte Lysate RNA polymerase Other buffer [35S]Met 30℃, 90mins
Binding GST protein Protein for translation GST-CA150WW2 protein GST-FBP11WW protein 30℃, 30mins
Pulled down Solution which made before beats 4℃, 2hours
cassette 2× screen film -70℃, 8hours Gel on paper
U2AF65 U2AF35 SF1 U35 10% input U65 10% input GST GST- CA GST- FB U2AF65 U2AF35?
U2AF35 U2AF35 U2AF65 SF1 U2AF65 U35 10% input U65 10% input GST- FB GST- CA GST GST GST- CA GST- FB U2AF35 ? U2AF65
A model fits my result
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