蛋白質交互作用資料庫、 網路拓樸分析與藥物標的搜尋 Protein Interactome, Topological Analysis on Complex Network for Identification of Drug Target http://hub.iis.sinica.edu.tw/ 分析流程圖/Hubba 透過Hubba/ Cytohubba 介面與平台，以拓樸分析找出複雜生物網路中的關鍵分子與交互次網路，並可利用此一介面，將與特定疾病或是調控機制相關的基因/蛋白質列表輸入，將可以找出與這些疾病或是調控機制相關，不在表列中的隱藏分子，這些分子將可作為新的藥物開發標的。 此外研究團隊亦將 Hubba 的分析功能整合成為 Cytoscape 的 plug-in – cytoHubba，以提供更高效能的分析。藉由 Cytoscape 的平台，不但可以很方便地分析蛋白質交互作用網路，也能進一步與其他 Cytoscape plugins 的功能整合。經由 Cytoscape 的輔助與其軟體能見度，cytoHubba 得以提供更多生物學家對龐大的基因網路，採取更精闢、便利的分析，進而提升生物網路分析的準確度! Here we also constructed a topological analyzer for complex network named as Hubba / cytohubba to identify the essential nodes and the relationships among them. Objectives of our work are to improve our understanding of the puzzle during development stage, carcinogenesis and infectious mechanism, and to furthermore introduce a new paradigm for the diagnosis and treatment of human disease to revolutionize current medical services delivered. Input format 輸入資料格式: PSI (1.5/ 2.0)/ XML、Tab delimitated、 Tab with weight 以多種演算法偵測網路中的 Hubs Hubs Identification by Several Algorithms Algorithms 請參閱演算法 Hubs 的第一層鄰居 The first –link of Hubs 前10名 Hubs 的子圖 Sub-graph by Top 10 hubs 特定 Hubs 的子圖 Sub-graph by specific Nodes CytoHubba http://hub.iis.sinica.edu.tw/cytohubba Top-ranked nodes, scored by selected method in cytoHubba Sub-network of top-ranked nodes with their expanded network The whole network 高血壓關連 基因表列 Hypertension related Genes: ACE ACE2 AGTR2 AGT BDKRB2 GHRL NTS AGTR1 ARRB2 人類蛋白質交互網路 Human Interactome 高血壓關連蛋白次網路 Sub network related with Hypertension Data panel for selected proteins with annotation 以整合式拓樸圖學演算法來重組複雜生物網路中的蛋白質複合體 Spotlight: Assembly of Protein Complexes by Integrating Graph Clustering Methods http://hub.iis.sinica.edu.tw/spotlight Refine Default 各類演算法與綜合結果分析 Select result by various topological methods. Or submit a list of nodes to find those complexes they involved. All the output can be opened in Cytoscape by submitted network and “CytoScape Node Attribute List” in the clustering result panel 輸入分析網路 Input Interactome in PSI/ Tab / Weighted Data 前十大複合體表列(by Score) Top 10 predicted complexes 點選任一複合體觀察其組成 Zoom in a complex by mouse double-click 酵母菌RNA聚合酶I, II, III型及其調控複合體 /RNA Polymerase I, II, III & Mediator in yeast 註解資料欄 /Annotation Functional Enrichment for predicted complex and each node 網頁圖像化呈現介面 Interactive and scalable network analyzer with various layouts 圖示說明/ Legends for those symbols in network 合作機構 Collaboration: 主持人： 林 仲彥 副研究員 Chung-Yen Lin Associate Research Fellow