The research of antagonistic organisms aganist phytopathogene 导师：戚春林老师 报告人：李卓阳

CONTENTS 01 02 03 PART ONE Introduction PART TWO Methods PART THREE Results

01 PART ONE Introduction

01 Introduction 01 In production agriculture, Fungal and bacterial plant pathogens infect plants and crops easily and often seriously, causing millions of dollars worth of economic damage each year. So, how to control these diseases and maintain the normal growth of plants is always the highlight.

01 Introduction 01 Application of chemical pesticides is the effective measure to control the fungal and bacterial infection and plays an important role in preventive defense. With the further knowledge to chemical pesticides, however, many concerns, such as the pollution of soil, water and atmosphere, the increase of the leftover of germicides in agricultural produce which imperils human health and life directly, the unbalance of ecological environment resulting from the pesticides, and resistance of phytopathogenic fungi and bacteria to germicides, have caused the withdrawal of many of them.

01 Introduction 01 Biological control by natural antagonistic organisms is a potential nonchemical tool for crop protection against phytopathogenic fungi and bacteria and offers a promising alternative to synthetic pesticides, in part because it is perceived as safe to the environment and human health and can avoid the appearance of drug-resistance.

02 PART TWO Methods

simple Antagonistic organisms 02 Methods 02 香蕉长形斑病病原菌(Curvularia fallax) 香蕉树木溃疡病病原菌(Botryosphaeria dothidea) 香蕉大灰斑病病原菌(Curvularia lunata) 胶孢炭疽菌(Colletotrichum gloeosporioides) 小麦赤霉病病原菌(FusaHum graminearum) 苹果轮纹病病原菌(Physalospora piricola) 黄瓜枯萎病病原菌(Fusarium oxysporum f. sp.cucumebrium) 香蕉枯萎病病原菌(Fusarium oxysporum f. sp. cubense).

02 Methods 02 Screening PROJECT Identification PROJECT Extraction 平板对峙法 琼脂孔扩散法 PROJECT Identification 形态特征 生理生化特征 16s rRNA PROJECT Extraction 验证 作用机理 耐受性 Results PROJECT

03 PART THREE Results

03 Results 03

03 Results 03

03 Results 03

03 Results 03

03 Results 03

Literature Cited 10 [1] 谢晶. 植物病原菌拮抗微生物的筛选、鉴定及拮抗机理研究[D].四川大学,2004. [2] 陈志杰,谢江辉,陈宇丰,周登博,高祝芬,戚春林.一株植物病原拮抗细菌的分离筛选及拮抗物质.生态学 杂志:1-14. [3] Chen Y, Zhou D, Luo Y, et al. Growth Promotion and Disease Suppression Ability of aStreptomycessp. CB-75 from Banana Rhizosphere Soil:[J]. Frontiers in Microbiology, 2017, 8:2704.

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